NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

HANC

Description

The SOPs contained in this compendium are a product of the Office of HIV/AIDS Network Coordination (HANC) (https://www.hanc.info/) and were developed by the AIDS Clinical Trials Group/International Maternal Pediatric Adolescent AIDS Clinical Trials group (ACTG/IMPAACT) Lab Tech Committee (LTC). Additional information on quality assurance guidance and providers; safety resources; and supportive documents such as instructions, evaluation forms, demonstrations, and checklists can also be found on the website. SOPs in this compendium are available at https://www.hanc.info/labs/labresources/procedures/Pages/actgImpaactLabManual.aspx.

Sources

  • HANC

SOPs

Title Version (Release Date) Source
Collection and Processing of Semen (SOP 015) HANC
Collection, Processing and Storage of Female Genital Secretions (SOP 016) HANC
Processing of Dried Blood Spots (SOP 55) HANC
Procedure for Serum Processing of Whole Blood Collected in Clot (NON) or SST Tube (SOP 66) HANC
Gut-Associated Lymphoid Tissue (GALT) Colorectal Biopsy: Collection and Initial Processing at Local ACTG Laboratories (SOP 69) HANC
Collection, Clinic Storage and Transport of Sputum (Expectorated) Specimens HANC
Lymph Node Biopsy Standard Operating Procedure (SOP 71) HANC
Adipose Tissue Biopsy Standard Operating Procedure (SOP 72) HANC
Sputum Processing for Storage and Tuberculosis Biomarker Research (SOP 074) HANC
Cerebral Spinal Fluid (SOP 67) HANC
Dried PBMC Pellet Processing Standard Operating Procedure (SOP 59) HANC
Cross- Network PBMC Processing SOP HANC
PBMC Isolation from Leukapheresis Standard Operating Procedure (SOP 61) HANC
Peripheral Blood Mononuclear Cell (PBMC) Thawing Standard Operating Procedure (SOP 65) HANC
Preparation of PHA-Stimulated Uninfected Donor Peripheral Blood Mononuclear Cells HANC
Umbilical Cord Blood Collection Standard Operating Procedure (SOP 63) HANC
HIV-1 PROVIRAL POLYMERASE CHAIN REACTION (HIV-1 DNA PCR) Using the Roche Amplicor HIV-1 Test HANC
IMPAACT and ACTG Cross-Network QFT-Plus Standard Operating Procedure HANC
Perkin Elmer/NEN Life Science Products HIV-1 p24 ELISA HANC
Microscope Slides and Paraffin Block Storage and Handling (LTC-SOP-075) HANC
Microbiome Sample Collection SOP: Stool collection and rectal swabs for gut microbiome analysis HANC
INTRACELLULAR Ki67 STAINING HANC
Flow Cytometry Immune Activation SOP HANC
Flow Cytometry SOP: Monocytes from Frozen Cells HANC
LYMPHOCYTE PROLIFERATION USING SUCCINIMIDYL ESTER OF CARBOXYFLIORESCEIN DIACETATE HANC
CFSE ISL SOP HANC
Celera ViroSeq™ HIV-1 Genotyping System HANC
Flow Cytometric quantitation of CD38 expression on CD8+ T lymphocytes HANC
Receptor Occupancy for ISL SOP/Methods HANC
Bayer HIV-1 TRUGENE Genotyping Method HANC
ACTG Consensus Method for 3-color Advanced Flow HANC
CTG/IMPAACT Network Guidelines for Shipping and Receiving Biological Substance, Category B specimens (LTC-SOP-22) HANC
Flow Cytometry SOP: 14 color flow for immune activation, senescence, and exhaustion HANC
Download all SOP documents of selected versions [33]

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