NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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CFSE ISL SOP   Add to my SOP compendium

BRD ID: 2,511

Source: HANC Tier: SOP Number of times downloaded: 179

Description

This document describes the procedures used by the Office of HIV/AIDS Network Coordination (HANC) for measuring proliferation of Peripheral Blood Mononuclear Cells (PBMCs) using carboxyfluorescein succinimidyl ester (CFSE) fluorescent dye and flow cytometry analysis of EDTA blood collected from clinical trial participants. It is developed and maintained by the AIDS Clinical Trials Group (ACTG)/International Maternal Pediatric Adolescent AIDS Clinical Trials group (IMPAACT) Lab Tech Committee (LTC) and is available under the heading "Other SOPs" at https://www.hanc.info/labs/labresources/procedures/Pages/actgImpaactLabManual.aspx.

Documents for Download

CFSE ISL SOP.pdf [Size: 125 KB]
Biospecimens
  • Fluid - Blood
  • Cell - White Cells
Diagnoses
  • AIDS/HIV-related
Topics
  • Platform-specific Methodology

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