NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Flow Cytometry Immune Activation SOP   Add to my SOP compendium

BRD ID: 2,514

Source: HANC Tier: SOP Number of times downloaded: 187

Description

This document describes the procedures used by the Office of HIV/AIDS Network Coordination (HANC) for measuring immune activation of T cells by flow cytometry using frozen Peripheral Blood Mononuclear Cells (PBMCs) isolated from heparin or EDTA blood collected from clinical trial participants. It is developed and maintained by the AIDS Clinical Trials Group (ACTG)/International Maternal Pediatric Adolescent AIDS Clinical Trials group (IMPAACT) Lab Tech Committee (LTC) and is available under the heading "Other SOPs" at https://www.hanc.info/labs/labresources/procedures/Pages/actgImpaactLabManual.aspx.

Documents for Download

Immune Activation Flow Cytometry SOP Final.pdf [Size: 578 KB]

Biospecimens
  • Fluid - Blood
  • Cell - White Cells
Diagnoses
  • AIDS/HIV-related
Topics
  • Platform-specific Methodology

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