NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

IMPAACT and ACTG Cross-Network QFT-Plus Standard Operating Procedure   Add to my SOP compendium

BRD ID: 2,519

Source: HANC Tier: SOP Number of times downloaded: 200

Description

This document describes procedures used by the Office of HIV/AIDS Network Coordination (HANC) for the collection of lithium heparin blood, the transfer of plasma to QFT-Plus tubes, and the processing, transport, storage, and analysis of specimens for IFN-γ levels by ELISA using the QIAGEN QuantiFERON-TB Gold Plus (QFT-Plus) Interferon Gamma Release Assay (IGRA). It is developed and maintained by the AIDS Clinical Trials Group (ACTG)/International Maternal Pediatric Adolescent AIDS Clinical Trials group (IMPAACT) Lab Tech Committee (LTC) and is available under the heading "Tuberculosis" at https://www.hanc.info/labs/labresources/procedures/Pages/actgImpaactLabManual.aspx.

Documents for Download

QFT Plus SOP_v1.0.pdf [Size: 650 KB]

Biospecimens
  • Fluid - Blood
  • Fluid - Plasma
Diagnoses
  • AIDS/HIV-related
Topics
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Storage
  • Platform-specific Methodology

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