LYMPHOCYTE PROLIFERATION USING SUCCINIMIDYL ESTER OF CARBOXYFLIORESCEIN DIACETATE
BRD ID: 2,512
Source: HANC Tier: SOP Number of times downloaded: 89
Description
This document describes procedures used by the Office of HIV/AIDS Network Coordination (HANC) for the isolation of Peripheral Blood Mononuclear Cells (PBMCs) from EDTA, ACD, or heparin blood collected from clinical trial participants and subsequent measurement of proliferation using the fluorescent succinimidyl ester of carboxyfluorsecein diacetate and flow cytometry analysis. It is developed and maintained by the AIDS Clinical Trials Group (ACTG)/International Maternal Pediatric Adolescent AIDS Clinical Trials group (IMPAACT) Lab Tech Committee (LTC) and is available under the heading "Immunology" at https://www.hanc.info/labs/labresources/procedures/Pages/actgImpaactLabManual.aspx.
Documents for Download
Dye Dilution (CFSE) Proliferation.pdf [Size: 389 KB]Biospecimens
- Fluid - Blood
- Cell - White Cells
Diagnoses
- AIDS/HIV-related
Topics
- Biospecimen Processing
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Platform-specific Methodology