Flow Cytometry SOP: Monocytes from Frozen Cells
BRD ID: 2,513
Source: HANC Tier: SOP Number of times downloaded: 142
Description
This document describes procedures used by the Office of HIV/AIDS Network Coordination (HANC) to quantify immune cells based on staining of immunological markers (CD3, CD4, CD8, HLA-DR, CD38, CD19, CD14, CD16, CD56, CCR2, CX3CR1, CCR5, CXCR4, CD11b, CD142, PDL-1, CD120a, CD120b, CD163, and CD62P) by flow cytometry in frozen peripheral blood mononuclear cells (PBMCs) isolated from heparin or EDTA blood collected from clinical trial participants. It is developed and maintained by the AIDS Clinical Trials Group (ACTG)/International Maternal Pediatric Adolescent AIDS Clinical Trials group (IMPAACT) Lab Tech Committee (LTC) and is available under the heading "Other SOPs" at https://www.hanc.info/labs/labresources/procedures/Pages/actgImpaactLabManual.aspx.
Documents for Download
Frozen Monocytes Flow Cytometry SOP.pdf [Size: 575 KB]Biospecimens
- Fluid - Blood
- Cell - White Cells
Diagnoses
- AIDS/HIV-related
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology