Flow Cytometry SOP: 14 color flow for immune activation, senescence, and exhaustion
BRD ID: 2,504
Source: HANC Tier: SOP Number of times downloaded: 657
Description
This document describes the procedures used by the Office of HIV/AIDS Network Coordination (HANC) to quantify immune cells based on staining of immunological markers (CD3, CD4, CD8, HLA-DR, CD38, CCR7, CD45RA, CD73, CD160, PD-1, TIM-3, TIGIT, and LAG-3) and flow cytometric analysis in frozen peripheral blood mononuclear cells isolated from heparin or EDTA blood collected from clinical trial participants. It is developed and maintained by the AIDS Clinical Trials Group (ACTG)/International Maternal Pediatric Adolescent AIDS Clinical Trials group (IMPAACT) Lab Tech Committee (LTC) and is available under the heading "Other SOPs" at https://www.hanc.info/labs/labresources/procedures/Pages/actgImpaactLabManual.aspx.
Documents for Download
14 Color Flow Cytometry SOP.pdf [Size: 672 KB]Biospecimens
- Fluid - Blood
- Cell - White Cells
Diagnoses
- AIDS/HIV-related
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology