NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Immune Tolerance Network (NIH)

Description

The SOPs contained in this compendium are a product of the Immune Tolerance Network (ITN), a collaborative network funded by the National Institute of Allergy and Infectious Diseases (NIAID) within the National Institutes of Health (NIH). Individual SOPs can be found at https://www.immunetolerance.org/researchers/lab-protocols, as can additional guidance on commonly used flow cytometry panels. Additional information on the ITN and their mission is provided on their homepage (https://www.immunetolerance.org/).

Sources

  • Immune Tolerance Network

SOPs

Title Version (Release Date) Source
Collection and Preservation of Kidney or Liver Transplant Biopsy for ITN Study Immune Tolerance Network
Isolation and Cryopreservation of PBMC – CPT without Plasma Collection Immune Tolerance Network
Total RNA Isolation From Blood Collected into ABI Tempus™ Whole Blood Collection tubes Immune Tolerance Network
Concentration of isolated RNA using Millipore Microcon Centrifugal DeviceYM-100 Immune Tolerance Network
Assessing the quality of RNA using the Agilent 2100 Bioanalyzer Immune Tolerance Network
In vitro Intracellular Cytokine Staining With and Without Stimulation Immune Tolerance Network
Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method) Immune Tolerance Network
FoxP3 Staining for Flow Cytometry Immune Tolerance Network
Separation and Freezing Technique for Lymph Node Mononuclear Cells from Donor Lymph Nodes at ITN Central Cell Processing Core Facility Immune Tolerance Network
Isolation and Cryopreservation of PBMCs - Ficoll Method without plasma collection Immune Tolerance Network
THREE COLOR FLOW CYTOMETRIC CROSSMATCH Immune Tolerance Network
Cell surface antibody staining for flow cytometry Immune Tolerance Network
Precursor Frequency Determination Using CFSE Dilution Assay and Quantification of Cytokine Secreting Cells Using Intracellular Cytokine Staining Immune Tolerance Network
DETECTION OF IFN- γ and IL-10 SECRETING CELLS BY ELISPOT Immune Tolerance Network
Surface Staining of Human Cells Immune Tolerance Network
LIVE/DEAD VIABILITY FOR CLINICAL SAMPLES Immune Tolerance Network
Lymphocyte Proliferation Immune Tolerance Network
Performing Alloreactive ELISPOT assays Immune Tolerance Network
Instructions for processing and freezing of the Cerebral Spinal Fluid (CSF) for future isolation of RNA Immune Tolerance Network
Method for thawing frozen cells Immune Tolerance Network
Separation and Freezing Technique for Spleen Mononuclear Cells from Donor Spleens at ITN Central Cell Processing Core Facility Immune Tolerance Network
Versalyse Whole Blood Staining Method Immune Tolerance Network
Synthesizing cDNA Using ThermoScript RT-PCR Kit in 96 Plate Format Immune Tolerance Network
Synthesizing cDNA Using ThermoScript RT-PCR Kit Immune Tolerance Network
Thawing of Frozen Cells Immune Tolerance Network
Whole Blood Basophil Activation Assay Immune Tolerance Network
Whole Blood Washing Method Immune Tolerance Network
TRIzol extraction of ITN RNALater tissue biopsies SOP Immune Tolerance Network
Download all SOP documents of selected versions [28]

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