Cell surface antibody staining for flow cytometry
BRD ID: 1,372
Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 1356
Description
This document describes procedures for cell surface immunofluorescent staining and measurement of cell viability with optional amine reactive dye prior to flow cytometric analysis. The described procedures are suitable for use with fresh or frozen peripheral blood mononuclear cells (PBMC) and lysed whole blood. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.
Documents for Download
SOP_Cell Surface Staining for Flow Cytometry FIN.pdf [Size: 81 KB]Biospecimens
- Cell - Blood
- Cell - White Cells
Diagnoses
- General
Topics
- Biospecimen Processing
Associated SOP Compendiums
- Biospecimen Processing