NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

THREE COLOR FLOW CYTOMETRIC CROSSMATCH   Add to my SOP compendium

BRD ID: 1,371

Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 2274

Description

This document describes the procedure for performing flow cytometric crossmatch (FCXM) using serum from potential transplant recipients and cells from donors which involves a 3-color staining method followed by flow cytometric analysis. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.

Documents for Download

SOP_3_color_flow cytometric crossmatch.pdf [Size: 125 KB]

Biospecimens
  • Cell - Blood
  • Cell - Lymph Node
  • Cell - Spleen
  • Fluid - Serum
Diagnoses
  • General
Topics
  • Biospecimen Processing

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