Separation and Freezing Technique for Spleen Mononuclear Cells from Donor Spleens at ITN Central Cell Processing Core Facility
BRD ID: 1,381
Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 1223
Description
This document describes procedures for for the isolation and cryopreservation of spleen mononuclear cells (SMNC) using Ficoll method. It also describes the analysis of SMNC for cell viability using a ViCell™ XR Viability Analyzer. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.
Documents for Download
SOP_Separation and Freezing Technique for Spleen Mononuclear Cells.pdf [Size: 106 KB]Biospecimens
- Cell - White Cells
- Tissue - Spleen
Diagnoses
- Normal
Topics
- Biospecimen Processing
- Biospecimen Preservation
Associated SOP Compendiums
- Biospecimen Processing
- Biospecimen Preservation