Precursor Frequency Determination Using CFSE Dilution Assay and Quantification of Cytokine Secreting Cells Using Intracellular Cytokine Staining
BRD ID: 1,373
Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 981
Description
This document describes procedures for preparation of frozen peripheral blood mononuclear cells (PBMC) for the carboxyfluorescein succinimidyl ester (CFSE) dilution assay and the intracellular cytokine staining (ICS) assay using flow cytometry. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.
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SOP_CFSE labeling of PBMCs and measurement of proliferation to peanut antigen.pdf [Size: 335 KB]Biospecimens
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- Biospecimen Processing
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