NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

In vitro Intracellular Cytokine Staining With and Without Stimulation   Add to my SOP compendium

BRD ID: 1,366

Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 1092

Description

This document describes procedures for processing whole blood collected in sodium heparin and EDTA tubes for staining with intracellular cytokine and surface marker antibodies prior to flow cytometric analysis. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.

Documents for Download

ITN_Protocol_RPCI-001.pdf [Size: 76 KB]

Biospecimens
  • Cell - General
  • Fluid - General
Diagnoses
  • General
Topics
  • Biospecimen Processing

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