NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method)   Add to my SOP compendium

BRD ID: 1,367

Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 1438

Description

This document describes procedures for processing and immunostaining of whole blood collected in sodium heparin and EDTA tubes for cell surface and intracellular markers for analysis by flow cytometry. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.

Documents for Download

ITN_Protocol_RPCI-004.pdf [Size: 39 KB]

Biospecimens
  • Cell - General
  • Fluid - General
Diagnoses
  • General
Topics
  • Biospecimen Processing

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