NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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FoxP3 Staining for Flow Cytometry   Add to my SOP compendium

BRD ID: 1,368

Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 1484

Description

This document describes procedures for the preparation of cells from whole blood using a washed blood lysis technique, frozen cells from whole blood, and frozen cells separated by Ficoll gradient technique for Foxp3 staining prior to flow cytometric analysis. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.

Documents for Download

ITN_Protocol_RPCI-006.pdf [Size: 131 KB]
Biospecimens
  • Cell - Blood
  • Fluid - Blood
Diagnoses
  • General
Topics
  • Biospecimen Processing
  • Storage

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