FoxP3 Staining for Flow Cytometry
BRD ID: 1,368
Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 1395
Description
This document describes procedures for the preparation of cells from whole blood using a washed blood lysis technique, frozen cells from whole blood, and frozen cells separated by Ficoll gradient technique for Foxp3 staining prior to flow cytometric analysis. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.
Documents for Download
ITN_Protocol_RPCI-006.pdf [Size: 131 KB]Biospecimens
- Cell - Blood
- Fluid - Blood
Diagnoses
- General
Topics
- Biospecimen Processing
- Storage
Associated SOP Compendiums
- Biospecimen Processing
- Storage