NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

DETECTION OF IFN- γ and IL-10 SECRETING CELLS BY ELISPOT   Add to my SOP compendium

BRD ID: 1,374

Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 1104

Description

This document describes procedures for detecting IFN-γ and IL-10 secreting cells by Enzyme-linked immunospot assay (ELISPOT) using frozen peripheral blood mononuclear cells (PBMC) collected from patients diagnosed with multiple sclerosis. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.

Documents for Download

SOP_Detection of IFNg and IL-10 secreting cells by ELISPOT.pdf [Size: 25 KB]

Biospecimens
  • Cell - White Cells
Diagnoses
  • Multiple Sclerosis
Topics
  • Biospecimen Processing

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