NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Lymphocyte Proliferation   Add to my SOP compendium

BRD ID: 1,377

Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 3011

Description

This document describes procedures for performing the Lymphocyte Proliferation Assay (LPA) using 3H-thymidine incorporation and calculating the stimulation index (SI) of peripheral blood mononuclear cells (PBMC) isolated from fresh heparinized blood. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.

Documents for Download

SOP_Lymphocyte Proliferation Assay using 3H-thymidine incorporation.pdf [Size: 87 KB]

Biospecimens
  • Cell - White Cells
Diagnoses
  • General
Topics
  • Biospecimen Processing

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