Lymphocyte Proliferation
BRD ID: 1,377
Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 3851
Description
This document describes procedures for performing the Lymphocyte Proliferation Assay (LPA) using 3H-thymidine incorporation and calculating the stimulation index (SI) of peripheral blood mononuclear cells (PBMC) isolated from fresh heparinized blood. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.
Documents for Download
SOP_Lymphocyte Proliferation Assay using 3H-thymidine incorporation.pdf [Size: 87 KB]Biospecimens
- Cell - White Cells
Diagnoses
- General
Topics
- Biospecimen Processing
Associated SOP Compendiums
- Biospecimen Processing