Isolation and Cryopreservation of PBMCs - Ficoll Method without plasma collection
BRD ID: 1,370
Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 2436
Description
This document describes procedures for isolation of peripheral blood mononuclear cells (PBMC) from blood collected in CPT tubes using the Ficoll method and their subsequent cryopreservation. Procedures on measuring cell counts using trypan blue and a hemacytometer are also described. It is a product of the Immune Tolerance Network (ITN; https://www.immunetolerance.org/). Additional ITN SOPs are available at https://www.immunetolerance.org/researchers/lab-protocols.
Documents for Download
Separation of mononuclear cells from whole blood using Ficoll gradients.pdf [Size: 460 KB]Biospecimens
- Cell - White Cells
- Fluid - Blood
Diagnoses
- General
Topics
- Biospecimen Processing
- Biospecimen Preservation
- Storage
Associated SOP Compendiums
- Biospecimen Processing
- Biospecimen Preservation
- Storage