NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

NCI Patient-Derived Models Repository

Description

The SOPs contained in this compendium are products of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR). The PDMR generates patient-derived models from primary and metastatic tumor tissues and blood specimens supplied by NCI-supported clinical trials and NCI-designated Cancer Centers and also accepts previously derived PDX models developed at external sites. The SOPs are available at https://pdmr.cancer.gov/sops/default.htm. To learn more about the PDMR and their mission please visit their homepage at https://pdmr.cancer.gov/default.htm.

Sources

  • PDMR

SOPs

Title Version (Release Date) Source
Fresh Tumor Collection and Handling for Generation of Patient-Derived Models PDMR
Blood Collection and Shipping for Isolation of Viable Circulating Tumor Cells PDMR
Patient Tumor Implantation (Subcutaneous) and Monitoring PDMR
PDX Implantation, Expansion and Cryopreservation (Subcutaneous) PDMR
Histopathological Assessment of Patient-Derived Xenografts PDMR
Recipes for Complete Media for Patient-Derived In Vitro and Organoid Cultures PDMR
Preparation of Matrigel-Coated Flasks for Adherent Patient-Derived In Vitro Cultures PDMR
Initial Culture, Sub-culture, and Cryopreservation of Adherent Patient-Derived Tumor Cultures (PDCs) PDMR
Initial Culture, Sub-culture, and Cryopreservation of Suspension Patient-Derived Tumor Cultures (PDCs) PDMR
Initial Culture and Sub-culture of Patient-Derived Cancer-Associated Fibroblasts (CAFs) PDMR
Thawing and Initial Culture of Patient-Derived Organoid (PDOrg) Cultures PDMR
Passaging and Sub-culture of Patient-Derived Organoid (PDOrg) Cultures PDMR
Cryopreservation of Patient-Derived Organoid (PDOrg) Cultures PDMR
Purification of Genomic DNA and Total RNA Tumor Tissue Using Qiagen AllPrep DNA/RNA Mini Kit PDMR
SureSelectXT Automated Target Enrichment for Illumina Paired-End Sequencing Library PDMR
Automated RNASeq Library Preparation using Illumina’s TruSeq RNA Access Library for Illumina Paired-End Sequencing PDMR
Illumina Sequencing Quantitation using Droplet Digital PCR (ddPCR) Protocol PDMR
Denaturing and Diluting Sequencing Library Pools and Cluster Generation Protocol PDMR
Illumina sequencing library pools on a HiSeq 2500 (external) PDMR
Shipping and Handling of Fresh Autopsy/Post-Mortem Specimens (non-frozen) for Generation of Preclinical Models PDMR
Shipping and Handling of Viably Cryopreserved Autopsy/Post-Mortem Specimens for Generation of Preclinical Models PDMR
Download all SOP documents of selected versions [21]

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