SureSelectXT Automated Target Enrichment for Illumina Paired-End Sequencing Library
BRD ID: 2,284
Source: PDMR Tier: SOP Number of times downloaded: 824
Description
This document describes the procedures used by the Molecular Characterization and Clinical Assay Development Laboratory (MoCha) for shearing DNA extracted from tumor tissue using a Covaris E220 Ultrasonicator and preparing a DNA library using the Agilent SureSelect XT Target Enrichment System at the Frederick National Laboratory for Cancer Research. It is a product of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR) and is available at https://pdmr.cancer.gov/sops/default.htm.
Documents for Download
MCCRD_SOP0005_PDX_Automated_SureSelect_Multiplex_LibraryPrep_Illumina.pdf [Size: 482 KB]Biospecimens
- Tissue - General
- Patient Derived Model - General
Diagnoses
- Neoplastic - Not specified
Topics
- Biospecimen Processing
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Platform-specific Methodology