NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Illumina Sequencing Quantitation using Droplet Digital PCR (ddPCR) Protocol   Add to my SOP compendium

BRD ID: 2,286

Source: PDMR Tier: SOP Number of times downloaded: 924

Description

This document describes the procedures used by the Molecular Characterization and Clinical Assay Development Laboratory (MoCha) to quantify DNA libraries by digital droplet PCR (ddPCR) prior to sequencing patient-derived xenograft specimens on the Illumina HiSeq 2500 at the Frederick National Laboratory for Cancer Research. It is a product of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR; https://dctd.cancer.gov/drug-discovery-development/reagents-materials/pdmr) and is available at https://dctd.cancer.gov/drug-discovery-development/reagents-materials/pdmr/using-pdmr/sops.

Documents for Download

MCCRD_SOP0007_PDX_ddPCR_Library_Quantification.pdf [Size: 208 KB]
Biospecimens
  • Patient Derived Model - General
Diagnoses
  • Neoplastic - Not specified
Topics
  • Platform-specific Methodology

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