PDX Implantation, Expansion and Cryopreservation (Subcutaneous)
BRD ID: 2,273
Source: PDMR Tier: SOP Number of times downloaded: 520
Description
This document describes the procedures used by the Biological Testing Branch for the preparation of fresh and cryopreserved human tumor tissues for subcutaneous implantation in mouse models to generate patient-derived xenografts and the subsequent tumor tissue growth monitoring, harvesting, and cryopreservation for future passaging in alternate host strains at NCI-Frederick, Frederick National Laboratory for Cancer Research. It is a product of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR) and is available at https://pdmr.cancer.gov/sops/default.htm.
Documents for Download
SOP50102_PDX_Methodology.pdf [Size: 483 KB]Biospecimens
- Tissue - General
- Patient Derived Model - General
Diagnoses
- Neoplastic - Not specified
Topics
- Biospecimen Processing
- Biospecimen Preservation
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Biospecimen Preservation
- Platform-specific Methodology