NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Illumina sequencing library pools on a HiSeq 2500 (external)   Add to my SOP compendium

BRD ID: 2,288

Source: PDMR Tier: SOP Number of times downloaded: 856

Description

This document describes the procedures for sequencing DNA library samples from patient-derived xenograft specimens using the Illumina HiSeq 2500 ultra-high-throughput sequencing system. It is used/performed by the Molecular Characterization and Clinical Assay Development Laboratory (MoCha) at the Frederick National Laboratory for Cancer Research. It is a product of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR) and is available at https://pdmr.cancer.gov/sops/default.htm.

Documents for Download

MCCRD_SOP0009_PDX_Illumina_Sequencing_library_pools_external.pdf [Size: 149 KB]

Biospecimens
  • Patient Derived Model - General
Diagnoses
  • Neoplastic - Not specified
Topics
  • Platform-specific Methodology

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