Initial Culture, Sub-culture, and Cryopreservation of Suspension Patient-Derived Tumor Cultures (PDCs)
BRD ID: 2,278
Source: PDMR Tier: SOP Number of times downloaded: 410
Description
This document describes the procedures used by the Biological Testing Branch (BTB) for establishing suspension primary cell cultures from cryopreserved patient-derived tumor tissue. It also includes steps for the expansion, subculture, and cryopreservation of patient-derived tumor cultures at NCI-Frederick, Frederick National Laboratory for Cancer Research. It is a product of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR) and is available at https://pdmr.cancer.gov/sops/default.htm.
Documents for Download
SOP30104_PDMR_Suspension_PDC_Methodology.pdf [Size: 982 KB]Biospecimens
- Patient Derived Model - General
Diagnoses
- Neoplastic - Not specified
Topics
- Biospecimen Processing
- Biospecimen Preservation
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Biospecimen Preservation
- Platform-specific Methodology