Automated RNASeq Library Preparation using Illumina’s TruSeq RNA Access Library for Illumina Paired-End Sequencing
BRD ID: 2,285
Source: PDMR Tier: SOP Number of times downloaded: 361
Description
This document describes the procedures used by the Molecular Characterization and Clinical Assay Development Laboratory (MoCha) for quantifying and assessing quality of RNA extracted from patient-derived xenograft specimens using the Advanced Analytical Technologies’ High Sensitivity RNA Analysis Kit and a fragment analyzer and preparation of a cDNA library using Illumina’s RNA Access Library Prep Kit at the Frederick National Laboratory for Cancer Research. It is a product of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR) and is available at https://pdmr.cancer.gov/sops/default.htm.
Documents for Download
MCCRD_SOP0006_PDX_Automated_RNAAccess_LibraryPrep_Illumina.pdf [Size: 569 KB]Biospecimens
- Patient Derived Model - General
Diagnoses
- Neoplastic - Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology