Initial Culture, Sub-culture, and Cryopreservation of Adherent Patient-Derived Tumor Cultures (PDCs)
BRD ID: 2,277
Source: PDMR Tier: SOP Number of times downloaded: 1380
Description
This document describes the procedures used by the Biological Testing Branch (BTB) for establishing adherent primary cell cultures from cryopreserved patient-derived tumor tissue at NCI-Frederick, Frederick National Laboratory for Cancer Research. It also includes step for the expansion, subculture, and cryopreservation of patient-derived tumor cultures. It is a product of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR) and is available at https://pdmr.cancer.gov/sops/default.htm.
Documents for Download
SOP30103_PDMR_Adherent_PDC_Methodology.pdf [Size: 998 KB]Biospecimens
- Patient Derived Model - General
Diagnoses
- Neoplastic - Not specified
Topics
- Biospecimen Processing
- Biospecimen Preservation
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Biospecimen Preservation
- Platform-specific Methodology