Denaturing and Diluting Sequencing Library Pools and Cluster Generation Protocol
BRD ID: 2,287
Source: PDMR Tier: SOP Number of times downloaded: 392
Description
This document describes the procedures used by the Molecular Characterization and Clinical Assay Development Laboratory (MoCha) for denaturing and diluting DNA library samples on a cBot system prior to sequencing patient-derived xenograft specimens on the Illumina HiSeq 2500 at the Frederick National Laboratory for Cancer Research. It is a product of the National Cancer Institute's (NCI) Patient-Derived Models Repository (PDMR) and is available at https://pdmr.cancer.gov/sops/default.htm.
Documents for Download
MCCRD_SOP0008_PDX_Sequencing_library_dilute_denature_cluster.pdf [Size: 97 KB]Biospecimens
- Patient Derived Model - General
Diagnoses
- Neoplastic - Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology