NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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NASA GeneLab Compendium

Description

The documents in this compendium are products of the GeneLab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview) of the National Aeronautics and Space Administration (NASA). The SOPs in this compendium were generated by the GeneLab Sequencing Group to standardize next-generation sequencing analysis of specimens collected in space upon their return to earth. The SOPs in this compendium are publicly available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing. Additional SOPs describing procedures for the isolation of nucleic acids from soil and other environmental sources are also available on their website.

Sources

  • NASA GeneLab

SOPs

Title Version (Release Date) Source
GeneLab SOP 1.1 Sample aliquoting, labeling and storage NASA GeneLab
GeneLab SOP 1.2 Frozen tissue cutting NASA GeneLab
GeneLab SOP 2.1 Tissue homogenization using Bullet Blender Gold bead beater NASA GeneLab
GeneLab SOP 2.2 Tissue homogenization using Polytron Rotor Stator Homogenizer NASA GeneLab
GeneLab SOP 3.1 Qiagen Allprep DNA_RNA Mini with Qiagen RNase free DNase set NASA GeneLab
GeneLab SOP 3.2 TRIzol RNA Extraction with QIAGEN RNase-Free DNase Set and QIAGEN Allprep NASA GeneLab
GeneLab SOP 3.3 Feces DNA Extraction using Maxwell RSC instrument with Purefood GMO kit NASA GeneLab
GeneLab SOP 3.5 RiboPure Blood Kit with DNase digestion NASA GeneLab
GeneLab SOP 4.1 RNA_DNA_miRNA_cDNA quantification using Qubit Fluorimeter NASA GeneLab
GeneLab SOP 4.2 QC genomic DNA NASA GeneLab
GeneLab SOP 4.3 Quality analysis of RNA using Agilent Bioanalyzer 2100 System NASA GeneLab
GeneLab SOP 5.1 TruSeq Stranded Total RNA EpMotion NASA GeneLab
GeneLab SOP 5.2 Controls and Spike-ins NASA GeneLab
GeneLab SOP 5.3 Illumina Nextera DNA Flex Manual Library Preparation NASA GeneLab
GeneLab SOP 5.4 QIAseq UPX 3' Transcriptome Library Prep NASA GeneLab
GeneLab SOP 6.1 Qiagility Library Quant NASA GeneLab
GeneLab SOP 6.2 Quant-iT PicoGreen dsDNA quantification of Illumina sequencing libraries NASA GeneLab
GeneLab SOP 6.3 Quality analysis of sequencing libraries using 4200 TapeStation System with D1000 reagent kit NASA GeneLab
GeneLab SOP 6.4 Normalizing sequencing libraries and pooling without the use of iSeq output NASA GeneLab
GeneLab SOP 6.5 Sequencing library QC, normalization and pooling using iSeq output NASA GeneLab
GeneLab SOP 6.6 Library manual cleanup NASA GeneLab
GeneLab SOP 6.7 Sequencing library quantification using QIAseq Library Quant Assay NASA GeneLab
GeneLab SOP 7.1 Setting up NovaSeq 6000 and iSeq 100 sequencers NASA GeneLab
GeneLab SOP 8.2 Generating iSeq HTStream output to calculate library pooling values NASA GeneLab
Download all SOP documents of selected versions [24]

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