GeneLab SOP 3.1 Qiagen Allprep DNA_RNA Mini with Qiagen RNase free DNase set
BRD ID: 3,225
Source: NASA GeneLab Tier: SOP Number of times downloaded: 83
Description
This SOP describes procedures for the isolation of RNA and DNA from tissues that were homogenized according to the protocols outlined in SOP #2.1 or #2.2 using the Qiagen AllPrep DNA/RNA Mini Kit; it was developed and is used by the National Aeronautics and Space Administration (NASA) GeneLab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview). The procedure includes a required DNase treatment step of isolated RNA and protocol adaptations for the isolation of nucleic acids from mouse skeletal muscle, heart, and skin. This SOP is a product of the NASA GeneLab and is available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing.
Documents for Download
GeneLab SOP 3.1 Qiagen Allprep DNA_RNA Mini with Qiagen RNase free DNase set.pdf [Size: 504 KB]Biospecimens
- Tissue - General
- Tissue - Heart
- Tissue - Skin
- Tissue - Muscle (Skeletal)
Diagnoses
- General
- Not specified
Topics
- Analyte Extraction and Purification
Associated SOP Compendiums
- Analyte Extraction and Purification