GeneLab SOP 2.1 Tissue homogenization using Bullet Blender Gold bead beater
BRD ID: 3,206
Source: NASA GeneLab Tier: SOP Number of times downloaded: 82
Description
This SOP describes the procedures for the homogenization of frozen tissues in lysis buffer (SOP #1.2 ) using a Bullet Blender 24 Gold bead beater prior to isolation of nucleic acids using Qiagen AllPrep Kits (SOP #3.1) or Trizol reagent (SOP #3.2); it was developed and is used by the National Aeronautics and Space Administration (NASA) GeneLab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview). The SOP includes homogenization settings for different tissue and bead (Next advanced NAVY, Zirconium Oxide 2.0 mm, Red or SSB kits) combinations. This SOP is a product of the NASA GeneLab and is available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing.
Documents for Download
GeneLab SOP 2.1 Tissue homogenization using Bullet Blender Gold bead beater.pdf [Size: 300 KB]Biospecimens
- Tissue - General
- Tissue - Thymus Gland
- Tissue - Liver
- Tissue - Colorectal
- Tissue - Lung
- Tissue - Spleen
- Tissue - Muscle (Skeletal)
- Tissue - Kidney
- Tissue - Heart
- Tissue - Brain
- Tissue - Lymph Node
- Tissue - Adipose
- Tissue - Adrenal Gland
Diagnoses
- General
- Not specified
Topics
- Analyte Extraction and Purification
Associated SOP Compendiums
- Analyte Extraction and Purification