NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

GeneLab SOP 1.2 Frozen tissue cutting   Add to my SOP compendium

BRD ID: 3,205

Source: NASA GeneLab Tier: SOP Number of times downloaded: 81

Description

This SOP describes procedures for sectioning frozen tissues and placing the sections in lysis buffer for homogenization (see SOP #2.1 or #2.2) that were developed by the National Aeronautics and Space Administration (NASA) Genelab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview). The SOP also includes the validated minimum weight required for nucleic acid extraction from different tissue types from a mouse model. This SOP is a product of the NASA GeneLab and is available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing.

Documents for Download

GeneLab SOP 1.2 Frozen tissue cutting.pdf [Size: 249 KB]

Biospecimens
  • Tissue - General
  • Tissue - Thymus Gland
  • Tissue - Liver
  • Tissue - Colorectal
  • Tissue - Lung
  • Tissue - Skin
  • Tissue - Spleen
  • Tissue - Muscle (Skeletal)
  • Tissue - Kidney
  • Tissue - Heart
  • Tissue - Lymph Node
  • Tissue - Brain
  • Tissue - Adipose
  • Tissue - Adrenal Gland
Diagnoses
  • General
  • Not specified
Topics
  • Biospecimen Processing
  • Analyte Extraction and Purification

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