GeneLab SOP 2.2 Tissue homogenization using Polytron Rotor Stator Homogenizer
BRD ID: 3,224
Source: NASA GeneLab Tier: SOP Number of times downloaded: 123
Description
This SOP describes the procedures for the homogenization of frozen tissues in lysis buffer (SOP #1.2) using a hand-held Polytron rotor stator homogenizer before nucleic acid isolation using Qiagen AllPrep Kits (SOP #3.1), Trizol reagent (SOP #3.2) or the MAXwell Feces Kit (SOP# 3.3). The procedure specified was developed by the National Aeronautics and Space Administration (NASA) GeneLab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview) and is used for mouse skin when larger volumes are required for lysis or in situations where the bead-based method has not been optimized. This SOP is a product of the NASA GeneLab and is available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing.
Documents for Download
GeneLab SOP 2.2 Tissue homogenization using Polytron Rotor Stator Homogenizer.pdf [Size: 280 KB]Biospecimens
- Tissue - Adipose
- Tissue - Colorectal
- Tissue - Thymus Gland
- Tissue - Liver
- Tissue - Lung
- Tissue - General
- Tissue - Skin
- Tissue - Spleen
- Tissue - Muscle (Skeletal)
- Tissue - Kidney
- Tissue - Brain
- Tissue - Heart
- Tissue - Lymph Node
- Tissue - Adrenal Gland
Diagnoses
- General
- Not specified
Topics
- Analyte Extraction and Purification
Associated SOP Compendiums
- Analyte Extraction and Purification