NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

GeneLab SOP 3.5 RiboPure Blood Kit with DNase digestion   Add to my SOP compendium

BRD ID: 3,227

Source: NASA GeneLab Tier: SOP Number of times downloaded: 63

Description

This SOP describes procedures for the isolation of RNA and DNA from tissues homogenized according to the protocols outlined in SOP #2.1 or #2.2 using TRIzol reagent, the purification of the isolated RNA using the Qiagen AllPrep Mini Kit, and the removal of DNA using the QIAGEN RNase-Free DNase Set. The SOP was developed by the National Aeronautics and Space Administration (NASA) GeneLab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview) and is available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing.

Documents for Download

GeneLab SOP 3.5 RiboPure Blood Kit with DNase digestion.pdf [Size: 309 KB]

Biospecimens
  • Fluid - Blood
Diagnoses
  • General
  • Not specified
Topics
  • Analyte Extraction and Purification

Associated SOP Compendiums

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