GeneLab SOP 8.2 Generating iSeq HTStream output to calculate library pooling values
BRD ID: 3,242
Source: NASA GeneLab Tier: SOP Number of times downloaded: 48
Description
This SOP describes the procedure for calculating volumes to pool next-generation sequencing libraries using data from the first iSeq 100 run; the protocol was developed by the National Aeronautics and Space Administration (NASA) GeneLab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview). The SOP also includes instructions for library pooling when there is an insufficient library volume and instructions on how to estimate NovaSeq reads based on iSeq outputs. The SOP is a product of the GeneLab Sequencing Group and is available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing.
Documents for Download
GeneLab SOP 8.2 Generating iSeq HTStream output to calculate library pooling values.pdf [Size: 430 KB]Biospecimens
- Cell - General
- Fluid - General
- Tissue - General
Diagnoses
- General
- Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology