NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

GeneLab SOP 6.5 Sequencing library QC, normalization and pooling using iSeq output   Add to my SOP compendium

BRD ID: 3,238

Source: NASA GeneLab Tier: SOP Number of times downloaded: 129

Description

This SOP describes the procedures for pooling, normalization, and quality verification of next-generation sequencing libraries using Agilent Tapestation and Illumina iSeq instruments and was developed by the National Aeronautics and Space Administration (NASA) GeneLab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview). Prior to pooling, the libraries undergo individual quality control using a TapeStation system. Libraries are then quantified and pooled using an Illumina iSeq instrument. The SOP is a product of the GeneLab Sequencing Group and is available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing.

Documents for Download

GeneLab SOP 6.5 Sequencing library QC, normalization and pooling using iSeq output.pdf [Size: 374 KB]

Biospecimens
  • Cell - General
  • Fluid - General
  • Tissue - General
Diagnoses
  • General
  • Not specified
Topics
  • Platform-specific Methodology

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