GeneLab SOP 6.5 Sequencing library QC, normalization and pooling using iSeq output
BRD ID: 3,238
Source: NASA GeneLab Tier: SOP Number of times downloaded: 129
Description
This SOP describes the procedures for pooling, normalization, and quality verification of next-generation sequencing libraries using Agilent Tapestation and Illumina iSeq instruments and was developed by the National Aeronautics and Space Administration (NASA) GeneLab Sequencing Group (https://genelab.nasa.gov/genelab-sequencing-services/overview). Prior to pooling, the libraries undergo individual quality control using a TapeStation system. Libraries are then quantified and pooled using an Illumina iSeq instrument. The SOP is a product of the GeneLab Sequencing Group and is available for download from https://genelab.nasa.gov/genelab-sequencing-services/sops and https://github.com/nasa/GeneLab-sampleProcessing.
Documents for Download
GeneLab SOP 6.5 Sequencing library QC, normalization and pooling using iSeq output.pdf [Size: 374 KB]Biospecimens
- Cell - General
- Fluid - General
- Tissue - General
Diagnoses
- General
- Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology