NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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HIMC Compendium

Description

This compendium includes SOPs from the Human Immune Monitoring Center (HIMC) at Stanford School of Medicine. The SOPs in this compendium can be found online at https://iti.stanford.edu/himc/protocols.html.

Sources

  • HIMC

SOPs

Title Version (Release Date) Source
Serum processing HIMC
Plasma processing-heparin HIMC
Plasma processing-EDTA HIMC
PBMC processing-heparin HIMC
PBMC processing-EDTA HIMC
PBMC processing using SepMate tubes HIMC
Collection of granulocytes for DNA isolation HIMC
Whole blood stimulation in cluster tubes HIMC
Preparing CPT tubes for shipping HIMC
PBMC cryopreservation (from spun CPT tubes) HIMC
PAXgene collection & storage HIMC
PAXgene RNA extraction HIMC
Trizol RNA extraction HIMC
Intracellular cytokine staining on PBMCs using CyTOFTM Mass Cytometry HIMC
Hemagglutinin inhibition (HAI) Assay HIMC
Intracellular cytokine staining on PBMCs HIMC
Luminex Multiplex Analysis HIMC
Phenotyping of Human PBMC using CyTOFTM Mass Cytometry HIMC
Phenotyping of Human PBMC using Lyoplate HIMC
TCR/BCR-Stimulated Phospho-Flow on PBMC HIMC
Cytokine-Stimulated Phosphoflow of PBMC using CyTOF Mass Cytometry HIMC
Cytokine-Stimulated Phosphoflow on PBMC HIMC
Cytokine-Stimulated Phosphoflow of Whole Blood using CyTOF Mass Cytometry HIMC
Download all SOP documents of selected versions [23]

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