NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

PBMC processing-EDTA   Add to my SOP compendium

BRD ID: 545

Source: HIMC Tier: SOP Number of times downloaded: 905

Description

This document describes the protocols used by the Human Immune Monitoring Center (HIMC) at Stanford School of Medicine for isolating peripheral blood mononuclear cells (PBMC) from EDTA blood using ficoll and for freezing and storing PBMC. It is a product of HIMC and is available at http://iti.stanford.edu/content/dam/sm/iti/documents/himc/protocols/SOP-PBMCDirectFicollEDTAv1-0.pdf

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SOP-PBMCDirectFicollEDTAv1-0.pdf [Size: 1009 KB]

Biospecimens
  • Fluid - Blood
  • Cell - White Cells
Diagnoses
  • General
Topics
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage

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