NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Intracellular cytokine staining on PBMCs   Add to my SOP compendium

BRD ID: 1,123

Source: HIMC Tier: SOP Number of times downloaded: 1584

Description

This document describes the protocols used by the Human Immune Monitoring Center (HIMC) at Stanford School of Medicine for processing peripheral bloods mononuclear cells (PBMCs) and intracellular cytokine staining for analysis by flow cytometry. It is a product of HIMC and is available at http://iti.stanford.edu/himc/protocols.html.

Documents for Download

ICSprotocol_042312.pdf [Size: 464 KB]
Biospecimens
  • Cell - White Cells
  • Fluid - Blood
Diagnoses
  • General
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

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