NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
Advanced SearchBrowse by AnalyteBrowse by Pre-analytical FactorSearch SOPsSOP Compendiums

Intracellular cytokine staining on PBMCs using CyTOFTM Mass Cytometry   Add to my SOP compendium

BRD ID: 1,121

Source: HIMC Tier: SOP Number of times downloaded: 1783

Description

This document describes the protocols used by the Human Immune Monitoring Center (HIMC) at Stanford School of Medicine for processing peripheral blood mononuclear cells (PBMCs) and intracellular cytokine staining for analysis by mass cytometry. It is a product of HIMC and is available at https://iti.stanford.edu/himc/protocols.html.

Documents for Download

CyTOFICS101013.pdf [Size: 344 KB]
Biospecimens
  • Cell - White Cells
  • Fluid - Blood
Diagnoses
  • General
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

Associated SOP Compendiums

News and Announcements

  • 2025’s Most Popular SOPs: See What Users Downloaded Most
  • Improving RNAseq Analysis of Small Biospecimens: New Recommendations Available
  • 2026 NCI Best Practices for Biospecimen Resources is Now Available!
    • More...