Cytokine-Stimulated Phosphoflow of PBMC using CyTOF Mass Cytometry
BRD ID: 1,128
Source: HIMC Tier: SOP Number of times downloaded: 778
Description
This document describes the protocols used by the Human Immune Monitoring Center (HIMC) at Stanford School of Medicine for cytokine stimulation of peripheral blood mononuclear cells (PBMCs) and staining with phospho-specific antibodies for analysis by mass cytometry. It is a product of HIMC and is available at http://iti.stanford.edu/himc/protocols.html.
Documents for Download
Phosphoflow-CyTOF-41014.pdf [Size: 69 KB]Biospecimens
- Cell - White Cells
Diagnoses
- General
Topics
- Biospecimen Processing
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Platform-specific Methodology