NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
Advanced SearchBrowse by AnalyteBrowse by Pre-analytical FactorSearch SOPsSOP Compendiums

Cytokine-Stimulated Phosphoflow on PBMC   Add to my SOP compendium

BRD ID: 1,129

Source: HIMC Tier: SOP Number of times downloaded: 932

Description

This document describes the protocols used by the Human Immune Monitoring Center (HIMC) at Stanford School of Medicine for cytokine stimulation of peripheral blood mononuclear cells (PBMCs) and staining with phospho-specific antibodies for analysis by flow cytometry. It is a product of HIMC and is available at https://iti.stanford.edu/himc/protocols.html.

Documents for Download

Phosphoflow-Cytokine050611.pdf [Size: 255 KB]
Biospecimens
  • Cell - White Cells
Diagnoses
  • General
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

Associated SOP Compendiums

News and Announcements

  • 2025’s Most Popular SOPs: See What Users Downloaded Most
  • Improving RNAseq Analysis of Small Biospecimens: New Recommendations Available
  • 2026 NCI Best Practices for Biospecimen Resources is Now Available!
    • More...