Cytokine-Stimulated Phosphoflow of Whole Blood using CyTOF Mass Cytometry
BRD ID: 1,130
Source: HIMC Tier: SOP Number of times downloaded: 1210
Description
This document describes the protocols used by the Human Immune Monitoring Center (HIMC) at Stanford School of Medicine for cytokine stimulation of peripheral blood mononuclear cells (PBMCs) in whole blood and staining with phospho-specific antibodies for analysis by mass cytometry. It is a product of HIMC and is available at http://iti.stanford.edu/himc/protocols.html.
Documents for Download
WholeBlood_Phosphoflow-CyTOF-41023.pdf [Size: 314 KB]Biospecimens
- Fluid - Blood
Diagnoses
- General
Topics
- Biospecimen Processing
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Platform-specific Methodology