NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Cytokine-Stimulated Phosphoflow of Whole Blood using CyTOF Mass Cytometry   Add to my SOP compendium

BRD ID: 1,130

Source: HIMC Tier: SOP Number of times downloaded: 1278

Description

This document describes the protocols used by the Human Immune Monitoring Center (HIMC) at Stanford School of Medicine for cytokine stimulation of peripheral blood mononuclear cells (PBMCs) in whole blood and staining with phospho-specific antibodies for analysis by mass cytometry. It is a product of HIMC and is available at http://iti.stanford.edu/himc/protocols.html.

Documents for Download

WholeBlood_Phosphoflow-CyTOF-41023.pdf [Size: 314 KB]
Biospecimens
  • Fluid - Blood
Diagnoses
  • General
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

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