NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Network for Pancreatic Organ Donors with Diabetes

Description

This compendium contains SOPs contributed by the Network for Pancreatic Organ Donors with Diabetes (nPOD) (https://www.jdrfnpod.org/). nPOD procures and characterizes pancreatic and related tissues (spleen, lymph node, pancreatic lymph node, peripheral blood, thymus, and bone marrow) from cadaver organ donors with type 1 diabetes. The SOPs in this compendium are available at https://www.jdrfnpod.org/for-investigators/standard-operating-procedures/.

Sources

  • nPOD

SOPs

Title Version (Release Date) Source
DNA EXTRACTION nPOD
SHIPPING FROM AUTOANTIBODY SCREENING LABORATORIES nPOD
IMMUNOPATHOLOGY nPOD
SAMPLE DISTRIBUTIONS nPOD
RNA EXTRACTION nPOD
HISTOLOGY nPOD
ISLET ISOLATION BY THE ISLET CORE LAB nPOD
CASE PROCESSING FOR ORGANS THAT WILL UNDERGO ISLET ISOLATION nPOD
PROTOCOL FOR SHIPPING HUMAN ISLET SUSPENSION nPOD
CASE PROCESSING nPOD
ISOLATION OF PBMC nPOD
ISOLATION OF CELLS FROM SPLEEN, THYMUS, AND LYMPH NODES nPOD
CRYOPRESERVED CELL THAWING nPOD
Download all SOP documents of selected versions [13]

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