NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

ISOLATION OF CELLS FROM SPLEEN, THYMUS, AND LYMPH NODES   Add to my SOP compendium

BRD ID: 2,253

Source: nPOD Tier: SOP Number of times downloaded: 1630

Description

This document describes the procedures used by Network for Pancreatic Organ Donors with Diabetes (nPOD) laboratories for isolating, freezing, and storing cells from fresh spleen, thymus, or lymph nodes. It includes steps for enumerating cells using an automated counter. It is a product of nPOD and is available for download at https://www.jdrfnpod.org/for-investigators/standard-operating-procedures/.

Documents for Download

SOP-60.1-Isolation_of_Cells_from_Spleen_Thymus_and_Lymph_Nodes_MP_IK-revision.pdf [Size: 2428 KB]

Biospecimens
  • Cell - Lymph Node
  • Cell - Pancreas
  • Cell - Spleen
  • Tissue - Lymph Node
  • Tissue - Pancreas
  • Tissue - Spleen
Diagnoses
  • Diabetes Type 1
Topics
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage

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