RNA EXTRACTION
BRD ID: 2,261
Source: nPOD Tier: SOP Number of times downloaded: 2511
Description
This document describes the procedures for RNA extraction from fresh, snap-frozen, and OCT-embedded frozen blocks that are used by Network for Pancreatic Organ Donors with Diabetes (nPOD) laboratories. It also includes steps for RNA quantification by spectrophotometry and quality assessment using a bioanalyzer. It is a product of nPOD and is available for download at https://www.jdrfnpod.org/for-investigators/standard-operating-procedures/.
Documents for Download
rna-extraction.pdf [Size: 154 KB]Biospecimens
- Tissue - Bone Marrow
- Tissue - Lymph Node
- Tissue - Pancreas
- Tissue - Spleen
- Tissue - Thymus Gland
Diagnoses
- Diabetes Type 1
Topics
- Biospecimen Processing
- Storage
- Analyte Extraction and Purification
Associated SOP Compendiums
- Biospecimen Processing
- Storage
- Analyte Extraction and Purification