NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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NCI BEBP: Cell-Free miRNA Blood Collection and Processing   Add to my SOP compendium

BRD ID: 2,824 Last Curated Date: 05-01-2023

Source: BBRB Tier: Biospecimen Evidence-Based Practices (BEBP) Number of times downloaded: 854

Description

This evidence-based, expert-vetted best practices document describes the collection and processing of blood for cell-free microRNA (miRNA, miR) analysis of human plasma and serum, and also includes guidance on miRNA extraction and sample/specimen storage. Biospecimens processed under these procedural guidelines are suitable for research and clinical analysis of circulating miRNA. This BEBP is intended to support the development of evidence-based Standard Operating Procedures (SOPs). The BEBP document series is a product of the National Cancer Institute's Biorepositories and Biospecimen Research Branch (NCI BBRB) and can also be found on their website (https://biospecimens.cancer.gov/resources/bebp.asp).

Documents for Download

Cell-Free miRNA Blood Collection and Processing BEBP.pdf [Size: 1208 KB]
Biospecimens
  • Fluid - Blood
  • Fluid - Plasma
  • Fluid - Serum
Diagnoses
  • General
  • Neoplastic
Topics
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage
  • Analyte Extraction and Purification
  • Preaquisition
  • Platform-specific Methodology

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