NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Collection of SOPs on Snap-Freezing Human Tissue Specimens using Dry Ice


The SOPs contained within this compendium pertain to snap-freezing human tissue biospecimens using dry ice. Each SOP is a product of a single source organization. The intent of this compendium is to facilitate comparisons of snap-freezing protocols across organizations. A Biospecimen Evidence-based Practice (BEBP) document on snap-freezing tissue specimens developed by the National Cancer Institute's Biorepositories and Biospecimen Research Branch (NCI BBRB) can be found at


  • MD Anderson CPDPC Consortium
  • nPOD
  • IARC
  • Pancreatic Cancer Europe
  • YCCI
  • NIH NeuroBioBank
  • HANC
  • GAPPS Repository
  • UCHC Tissue Repository
  • CC GMI
  • DCTD
  • BBRB


Title Version (Release Date) Source
CPDPC16-02 PROCEED Biospecimen Collection Methods MD Anderson CPDPC Consortium
Common Minimum Technical Standards and Protocols for Biological Resource Centres dedicated to Cancer Research IARC
Standard Operating Procedure (SOP) for Pancreatic Cancer Fresh Tissue samples Pancreatic Cancer Europe
Tissue processing YCCI
Brain Sectioning Protocols NIH NeuroBioBank
Adipose Tissue Biopsy Standard Operating Procedure (SOP 72) HANC
Lymph Node Biopsy Standard Operating Procedure (SOP 71) HANC
Gut-Associated Lymphoid Tissue (GALT) Colorectal Biopsy: Collection and Initial Processing at Local ACTG Laboratories (SOP 69) HANC
Placenta Collection GAPPS Repository
Tissue Processing UCHC Tissue Repository
Collection of Small Tissue Biopsies for Frozen Sectioning CC GMI
Tumor Frozen Needle Biopsy Specimen Collection and Handling DCTD
GTEx Work Instruction for Collection of Tissue and use of Dry Ice Prior to Storage BBRB
Download all SOP documents of selected versions [14]

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