NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Immune Tolerance Network (ITN) Clinical Trial Protocols

Description

The SOPs contained in this compendium are in active use in Immune Tolerance Network (ITN), clinical trials. The ITN is a collaborative network funded by the National Institute of Allergy and Infectious Diseases (NIAID) within the National Institutes of Health (NIH). Individual SOPs can be found at https://www.immunetolerance.org/researchers/lab-protocols, as can additional guidance on commonly used flow cytometry panels. Additional information on the ITN and their mission is provided on their homepage (https://www.immunetolerance.org/). An additional ITN SOP Compendium can be found at https://brd.nci.nih.gov/brd/sop-compendium/show/641.

Sources

  • Immune Tolerance Network

SOPs

Title Version (Release Date) Source
Collection and Cryopreservation of Lymphocytes from Lymph Node or Splenocytes from Spleen Immune Tolerance Network
On-site PBMC Isolation Using SepMate Tubes Standard Operating Procedure Immune Tolerance Network
Preparation of Platelet-Poor Plasma from Sodium Citrate Tubes Standard Operating Procedure Immune Tolerance Network
Renal Biopsy Processing for Single Cell Analyses Standard Operating Procedures Immune Tolerance Network
Urine Processing for Single Cell Analysis and Proteomics Standard Operating Procedure Immune Tolerance Network
Whole Blood Preservation with Smart Tubes Standard Operating Procedures Immune Tolerance Network
Microbiome viability assay Immune Tolerance Network
C4D Abcam - Paraffin AEC - Manual Immune Tolerance Network
C4D Frozen-FITC (1 Day Protocol) Immune Tolerance Network
H/E Frozen Protocol Immune Tolerance Network
H/E Paraffin Protocol Immune Tolerance Network
PAS Immune Tolerance Network
Periodic Acid Methenamine Silver (PAMS) Immune Tolerance Network
Richard Allan Scientific Methenamine Silver Trichome Kit Immune Tolerance Network
Masson Trichrome for FFPE Immune Tolerance Network
Diabetes Diagnostic Lab - C- peptide II (C-PEPII) Assay - Standard (ITN_C-peptide 5-2-22 SOP) Immune Tolerance Network
Human Recombinant GAD-65 In Vitro Translation & Autoantibody Immuno-Radioassay Protocol Immune Tolerance Network
Human Recombinant IA-2 In Vitro Translation & Autoantibody immuno-Radioassay Immune Tolerance Network
Human Recombinant Insulin Autoantibody Immuno-Radioassay Immune Tolerance Network
Human Recombinant ZnT8 in Vitro Translation & Autoantibody Immuno-Radioassay Immune Tolerance Network
Cytomegalovirus DNA Detection and Quantification Protocol By Light Cyler-PCR with Hybridization Probes Immune Tolerance Network
Epstein-Barr Virus DNA Detection and Quantification Protocol by Light Cycler-PCR with Roche Primers and Probes Kit Immune Tolerance Network
Collection and Preservation of Kidney or Liver Transplant Biopsy for ITN Study Immune Tolerance Network
1000-1136 Isolation and Cryopreservation of PBMC from whole blood Immune Tolerance Network
Download all SOP documents of selected versions [24]

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