OSUCCC Leukemia Tissue Bank

Version (Release Date):

Description

The SOPs contained in this compendium are a product of the OSUCCC Leukemia Tissue Bank (http://cancer.osu.edu/research-and-education/shared-resources/leukemia-tissue-bank).

Sources

OSUCCC LTB

SOPs

Title	Version (Release Date)	Source
Establishment of Primary Cell culture of human bone marrow aspirate: Culture and Harvest of Fibroblasts Cells	Version	OSUCCC LTB
Procedure to thaw cryopreserved mononuclear cells from bone marrow, peripheral blood or products of leukapheresis	Version	OSUCCC LTB
Mononuclear cell separation of bone marrow aspirate and peripheral blood using Ficoll-paque Plus	Version	OSUCCC LTB
Cell suspension enumeration and viability determination using the	Version	OSUCCC LTB
Non-enzymatic Single-Cell Dispersion of normal or tumor tissue	Version	OSUCCC LTB
Buccal Swab DNA Isolation with DNeasy Kit	Version	OSUCCC LTB
CD138 Positive Selection of Ficolled Mononuclear Cell Fraction from bone marrow or whole blood	Version	OSUCCC LTB
Preparation of cytological slides from mononuclear cell fraction of	Version	OSUCCC LTB
Preparation of plasma and serum from peripheral blood	Version	OSUCCC LTB
Banking Procedure for Previously Prepared Samples	Version	OSUCCC LTB
Quality Control of DNA using agarose gel electrophoresis with post-run staining	Version	OSUCCC LTB
Preparation of RNA lysate from mononuclear cell fraction	Version	OSUCCC LTB
Calibration of ViCell® Cell Counter using Via™Check Concentration Control	Version	OSUCCC LTB
Wright-Giemsa Stain of cytological “cytospin” slide preparations to determine percent blasts of leukemic blood or bone marrow aspirate sample after ficoll separation	Version	OSUCCC LTB

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