NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Procedure to thaw cryopreserved mononuclear cells from bone marrow, peripheral blood or products of leukapheresis   Add to my SOP compendium

BRD ID: 1,082

Source: OSUCCC LTB Tier: SOP Number of times downloaded: 711

Description

This document describes the procedures used by the OSUCCC Leukemia Tissue Bank to thaw cryopreserved cells isolated from peripheral blood, bone marrow aspirate, a single-cell suspension of a tissue sample, or from products of leukapheresis. Procedures describing cell viability testing using the trypan blue dye exclusion method are also provided. It is a product of the OSUCCC Leukemia Tissue Bank (http://cancer.osu.edu/research-and-education/shared-resources/leukemia-tissue-bank).

Documents for Download

OSU LTB Sample Thaw Procedure_hemacytometer count.v. 1.3.pdf [Size: 187 KB]

Biospecimens
  • Cell - White Cells
Diagnoses
  • General
Topics
  • Biospecimen Processing

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